Meanwhile, polyclonal antibody was purified by affinity chromatography and ammonium sulfate precipitation in this article and the antibody titer was detected by indirect ELISA. The purified antibody titers were respectively 1:16000 and 1:32000. 同时,通过亲和层析法和饱和硫酸铵法纯化的抗体经间接ELISA法检测,其效价分别为1:16000和1:32000。